The increasing use of fossil fuels, fluctuations in the price of petroleum products, the reduction of available reserves and the issue of environmental pollutants have encouraged researchers to find renewable and preferably non-fossil sources for energy supply. In the last decade, the use of plant tissue fuels, which have less pollution, have received much attention from industry and researchers. Methanol and ethanol can also be obtained from crude oil and natural gas, but ethanol is more important because it is easily renewable and from organic materials such as plant seeds or sugar beets, etc. are also available. Ethanol is flammable and ignites much better than other fuels. When ethanol is completely burned, the result is only water and carbon dioxide. Due to this issue, it is of interest in terms of reducing environmental pollutants and it is considered a suitable fuel for the public transportation sector. In this article, gasoline fuel with 96% ethanol was prepared as three mixtures of E5, E10 and E15 and will be analyzed in the EF7 engine. Also, the obtained results were compared with a similar study in which 99.8% ethanol was used to prepare mixed fuel. The results showed that E15 mixed fuel, which is prepared from ethanol with a concentration of 96%, increases the performance of the engine. Also, the maximum amount of torque occurred at 3000 rpm and is equal to 118.2 Nm, which shows that it has increased by 62.4% compared to special gasoline. Also, the obtained results showed that the use of ethanol with a higher percentage reduces engine performance.